Genetic Lab Report Example | Topics and Well Written Essays - 1500 words

Genetic - Lab Report ExampleThe effects of other processes, such as genetic imprinting, atomic number 18 important in determining the traits for other characteristics of the organism.Sequencing the genetic material of a species, and determining all coding sequences and their corresponding proteins are vital projects in the celestial orbit of molecular biology. The human genome project had been successful in sequencing the DNA of humans (Venter et al., cc1). The studies of (Kyrylkova et al., 2012 Yu et al., 2012) determine the characteristics corresponding to a particular genetic sequence by preventing the placement of the latter and observing the changes afterward. A sequence identified to determine a particular characteristic can also be used as a molecular marker to determine the presence or absence of trait, even without actually seeing it in the organism. For example, markers for sex can help distinguish surrounded by males and females in monomorphic species or their young.Th e objective of this experiment was to demonstrate the use of DNA samples to determine the characteristics of the organism, particularly its sex. Specifically, the activity aimed to isolate DNA from different sources, blood, muscle and feather. subsequently purification, the DNA isolates underwent polymerase chain reaction (PCR) using sexing primers 2250F and 2718R to replicate the sequence for sex determination. Agarose gel electrophoresis of the PCR products were compared with that of known male and female samples to identify the sex of the yellowed from which the samples were obtained.Qiagen DNeasy Blood and Tissue Extraction Kit was used for extraction. Briefly, the tissues sample was lysed by incubating it in a solution containing 20 l proteinase K, 4 l RNAse A and 166 l phosphate buffer solution (PBS) (blood) or 180 l Buffer ATL (muscle or feather) for 30 min. After mixing with 200 l Buffer AL for 15 sec, the DNA was precipitated by addition of 200 l 95%